Figure 7.

MGO modifies the interaction between hSlo1 and LRRC26. A and B: representative BK channel currents evoked from Xenopus oocytes expressing hSlo1 and LRRC26 (A) or hSlo1 alone (B) at +180 mV. Each trace is the average of 6 sweeps (acquired over 3 min). Outward current during the pulse as well as the inward tail current following the pulse decrease during MGO (5 mM) treatment (red) for channels containing LRRC26 (A), but not for Slo1 alone (B). Lower panels show kinetic changes in normalized tail currents. C: time course of MGO action. Data shown as means ± SE for 3 experiments each in the presence and absence of LRRC26 with each point representing the average of 3 sweeps (acquired over 90 s) at +180 mV. D: conductance-voltage (G_K-V) relations, normalized to +180 mV from the experiments in A and B, show MGO produces a positive shift in the voltage dependence of activation. BK, large conductance, Ca²⁺-activated, and voltage-dependent K⁺; MGO, methylglyoxal.