Skip to main content
. Author manuscript; available in PMC: 2023 Mar 27.
Published in final edited form as: Cell Rep. 2021 Nov 23;37(8):110034. doi: 10.1016/j.celrep.2021.110034

Figure 3. Top1 sumoylation in ulp1-ΔN and siz2Δ.

Figure 3.

(A) Relative levels of sumoylated Top1 evaluated by the SUMO assay. SMT3 (gene coding for yeast SUMO) and TOP1 were tagged at genomic loci. Asterisks indicate unmodified Top1-13Myc non-specifically bound to Ni-NTA beads. Tdp1-AID*- HA was depleted by 6 h auxin treatment (note: Tdp1 is 6HA-tagged in tdp1-AID background only).

(B) Sumoylation status of Top1-13Myc or Top1-4KR-13Myc mutants. The SUMO assay was performed as in (A). TOP1-13Myc or top1-4KR-13Myc (K65R, K91R, K92R, K600R) replace the genomic copy of TOP1.

(C) CPT sensitivity of non-sumoylated Top1 mutants. Spot assays were plated on YEPD media and imaged 48 h post-plating.