Extended Data Fig. 2. The function of KAT8 on immune regulation relies on PD1-PD-L1 axis.
a, Wild type (control), PD-L1 knockout (KO) and PD-L1 overexpression (OE) A375 cells were transfected with sgNC or sgRNAs targeting KAT8. Then cells were cocultured with activated T cells from human PBMCs in 48-well plates for 24 h. Then, the T cells were carefully washed away with PBS, and the adherent tumor cells were stained with crystal violet. n = 4 biologically independent experiments. p values were calculated by two-tailed Student’s t-test. Error bars indicate the mean ± SD. b,c, The indicated stable expression LLC1 cell lines were subcutaneously injected into mice. Tumor sizes were measured at the indicated time points (b), and the endpoint tumor weights were measured (c). n = 6 mice per group. p values in (b) were calculated by two-way ANOVA with Tukey multiple comparisons test. p values in (c) were calculated by two-tailed Student’s t-test. Error bars indicate the mean ± SD.