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. 2023 Mar 9;4(3):382–400. doi: 10.1038/s43018-023-00522-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Structured illumination microscopy (SIM) analysis of mEGFP-KAT8 and IRF1-mCherry localization in 143B cells. b, Live-cell images of 143B cells expressing mEGFP-KAT8 and IRF1-mCherry with DiD staining. c, 143B cells expressing TagBFP-KAT8 and IRF1-mCherry were stained with anti-fibrillarin, anti-coilin or anti-PML antibodies. d, Indicated purified proteins used for in vitro LLPS assays were analyzed by SDS-PAGE and stained with Coomassie blue. e, Droplet formation was analyzed in purified mEGFP-KAT8 WT/C316S, IRF1-mCherry at room temperature in the presence of 150 mM NaCl and 10% PEG 8000. The concentration of each protein was 10 μM. Hoechst 33342 was used for staining nuclei. Scale bars in a,c,e indicate 5 μm. Scale bar in b indicates 10 μm. The experiments in a-e were repeated three times with similar results.