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. 2023 Mar 27;13:4935. doi: 10.1038/s41598-023-32111-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stx1 production by strains H19 and 11368 in the presence of 2DPy, MMC or both. The Stx1 production levels of strains H19 (ST21C1) and 11368 (ST21C2) were determined. After adding each reagent to cultures grown to mid-log phase (T = 0 h), an aliquot of culture was collected every hour for 6 h to prepare cell lysates and their supernatants. Stx1 concentrations are shown as the mean values with standard deviations of biological triplicates.