Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 27;14:1705. doi: 10.1038/s41467-023-37215-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Kinetic modeling of deglycosylation of (a) Fc_WT and (b) IgG_WT by EndoS_WT and (c) IgG_WT by EndoS2_WT. LC-MS kinetic analysis of deglycosylation of Fc_WT and IgG_WT by EndoS_WT showing the hydrolysis over time of the two N-glycans linked to Asn297. The deglycosylation process occurs sequentially as seen by the initial increase then decrease in monoglycosylated population (dark green) over time while the diglycosylated (purple) and deglycosylated (orange) species increase over time. d Schematic representation of Fc N-glycan processing by EndoS. In a first step, EndoS binds the diglycosylated Fc and hydrolyzes one of the N-glycan of the Fc which causes the release of monoglycosylated Fc. In a second step, EndoS engages the monoglycosylated Fc and hydrolyzes the remaining N-glycan of the Fc to produced completely deglycosylated Fc.