Fig. 2.

Cardiac differentiation of MYH6-mScarlet cardiac iPSC reporter line confirming the reporter activity. A Schematic representation of the cardiac small molecule differentiation protocol used in the study. B MYH6-mScarlet reporter expression profile monitored during CM differentiation course by a time-lapse imaging system; scale bar is 100 μm. C Flow cytometry analysis of the reporter D103-4 hiPSC line at day 15 of differentiation showing the percentage of troponin-positive CMs that are mScarlet-positive confirming the cardiac-specific expression of the reporter. Troponin-positive cells were gated from the mixed population and were analyzed for the expression of mScarlet reporter. CHIR, glycogen synthase kinase 3β inhibitor-CHIR 9902; IWP4, inhibitor of WNT production-4