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. 2022 Jul 23;39(1):145–163. doi: 10.1007/s10565-022-09742-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Immunofluorescence images of MYH6-mScarlet reporter CMs expressing cardiac structural markers. Confocal image of fixed reporter CMs showing the myofibril striations (A). Reporter CMs immunostained with cardiac troponin T (cTnT) green (B), myosin-binding protein (MYBPC) green (C), and gap junction connexin 43 (Cx43) green (D). Nuclei were counterstained with DAPI (blue) in all immunostained images. Scale bars are 20 μm. Single/selective plane illumination microscopy (SPIM) optical 3D stack of multi-focal mScarlet fluorescence showing the spatial distribution (E) and topography of cardiomyocytes in an aggregate within cluster (F)