Fig. 3.

The cGAS-STING-IRF3/NF-κB pathway was activated in hearts of HFD-fed db/db mice. A The protein levels of cGAS, STING, p-IRF3/IRF3, p-p65/p65, and IL-1β in mouse myocardium of each group (n = 6, *P < 0.05, ***P < 0.001 vs db/ + group, using unpaired two-tailed t test). B–E Detection and quantification of cGAS, STING, NF-κB, and IRF3 in mouse heart of each group by immunofluorescence. The arrow indicated the activated NF-κB and IRF3. The Mander’s tM1 indicated that NF-κB or IRF3 co-localized with nucleus, whereas the Mander’s tM2 indicated nucleus that co-localized with NF-κB or IRF3 (n = 4, *P < 0.05, **P < 0.005, ***P < 0.001 vs db/ + group, using unpaired two-tailed t test). F, G Relative mRNA level of cGAS, STING, IL-1β, and IL-18 in mouse myocardium of each group (n = 6, ***P < 0.001 vs db/ + group, using unpaired two-tailed t test)