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. 2023 Feb 14;50(4):3557–3568. doi: 10.1007/s11033-023-08259-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Silencing of lncRNA PCAT6 inhibited LUAD cell growth, migration, invasion and EMT (a) RT‑qPCR analysis of the transfection efficiency of three si-PCAT6 sequences. (b-c) EdU assays and CCK8 were performed to examin A549 and H1299 cell growth. (d-e) Scratch assays and transwell assays were performed to examine the effect of silencing of PCAT6 on LUAD cells. (f) WB assays tested the protein levels of EMT-related protein. *P < 0.05, **P < 0.01, ***P < 0.001 vs. si-NC group