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. 2023 Mar 27;10:167. doi: 10.1038/s41597-023-02074-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Pseudotime trajectory analysis. (a) Distribution of query T cells on ProjecTILs reference map. (b) Percentage and number of T cells from each functional state. (c) UMAP plot of CD8+ T cell subset colored by pseudotime (top) and boxplot showing median pseudotime of each cell type (bottom). (d) UMAP plot of cancer cell subset colored by pseudotime (top) and boxplot showing median pseudotime of each cell type (bottom). (e) Chosen genes of the CD8+ T cell subset showing changing expression in pseudotime. (f) Chosen genes of the cancer cell subset showing changing expression in pseudotime.