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. 2022 Oct 13;44(4):766–779. doi: 10.1038/s41401-022-01000-7

Fig. 2. Activation of TRPV1 enhances remyelination.

Fig. 2

a Scheme of capsaicin treatment in the CPZ-induced model. b Rotarod test and beam walking test graphs demonstrate the motor coordinative function of WT mice treated with different doses of capsaicin: CAP-L, CAP-M, and CAP-H represent 1 mg/kg, 2 mg/kg, and 5 mg/kg of capsaicin i.p injection, respectively (mean ± SEM; n = 8 mice for each group; *P < 0.05, ***P < 0.001 by two-way ANOVA with Sidak’s multiple comparisons test). c The mRNA expression of genes related to mature oligodendrocyte/myelin (left panel) or oligodendrocyte lineage (right panel) was analyzed using RT-PCR; the color represents the value of the relative expression of a gene in a sample compared to the average value of this gene expression of the CPZ group; bright red represents a higher value, whereas a lower value is close to white color (mean ± SEM; n = 3 mice for each group). d Representative immunofluorescent images of mature oligodendrocytes (CC1+) in the corpus callosum. e Quantification of CC1+ mature oligodendrocytes (mean ± SEM; n = 3 mice for each group, scale bar = 100 μm, WT CPZ + CAP vs. WT CPZ, Trpv1−/− CPZ vs. WT CPZ, *P < 0.05 by unpaired two-tailed t-test) in d. f Western blotting analysis of MBP protein expression (normalized with β-actin expression) in the corpus callosum at the end of capsaicin treatment. g The quantification of MBP expression (mean ± SEM; n = 4 mice for each group; *P < 0.05 by one-way ANOVA with Tukey’s multiple comparisons test) in f. h Representative immunofluorescent images of MBP staining in the corpus callosum (scale bar = 50 μm). i Representative TEM images showing the ultrastructure of myelin/axons in the corpus callosum (scale bar = 5 μm). j Quantification of myelinated axons (mean ± SEM; n = 3 randomly selected areas from one sample of each group; *P < 0.05, **P < 0.01 by one-way ANOVA with Tukey’s multiple comparisons test) in i. k Motor coordinative function of Trpv1−/− mice treated with 2 mg/kg capsaicin was analyzed by rotarod test and beam walking test; (mean ± SEM; n = 6–7 mice for each group; **P < 0.01, ***P < 0.001 by two-way ANOVA with Tukey’s multiple comparisons test).