Table 2.

Plasmids used in this study.

Code	Plasmid description	references
pFL038	CEN6 URA3	27
pMEL10	2 μm ampR KlURA3 gRNA-CAN1.Y	19
Cas9-NAT	CEN6 TEF1-Cas9 natMX6	28
pGK044	CEN6 QDR3-kanMX-URA3	This study