Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 27;14:1710. doi: 10.1038/s41467-023-37419-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schemes of early-Abx mouse model experimental design. b Representative FACS plots and bar graphs for the percentages and absolute number of LrNK cells from control and early-Abx mice (weaning mice: n = 6 per group; 8-week-old mice: control n = 6, early-Abx n = 7). c, d Representative FACS plots and bar graph for the expression level (MFI) of CD11b, CD27 and KLRG1 in LrNK cell subsets from weaning or adult control and early-Abx mice (weaning mice: control n = 6, early-Abx n = 6; 8-week-old mice: control n = 6, early-Abx n = 7). e, f Representative FACS plots bar graph and for the expression (MFI) of effector molecules in PMA and Ion-stimulated LrNK cells from weaning or adult control and early-Abx mice (weaning mice: n = 6 per group; 8-week-old mice: control n = 6, early-Abx n = 7). g Representative FACS plots and bar graph for the expression (MFI) of IFN-γ expression in IL-12/15 or poly(I:C) - stimulated LrNK cells from 8-week-old control and early-Abx mice (n = 6 per group). h Cytotoxicity of LrNK cells against CFSE-labeled YAC-1 cells. 7-AAD⁺ CFSE⁺ cells represented the killed cells (control n = 6, early-Abx n = 8). i Representative FACS plots and bar graph for the expression (MFI) of Tim-3, NKG2A and PD-1 in LrNK cells from control and early-Abx mice (control n = 6, early-Abx n = 7). Each symbol represents data from an individual mouse, and error bars represent SEM per group in one experiment. Data were analyzed using two-tailed Student’s t-test. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001; ns, no significance. All experiments were repeated two or three independent experiments. Source data are provided as a Source Data file.