FIGURE 2.

Contributions of age, sex and Magel2-KO genotype on physiological brain regional OXTR expression levels. Bar graphs of OXTR levels quantified by [¹²⁵I]-OVTA binding in P8 and P90, male and female, WT and Magel2-KO mice. Each histogram represents data expressed as mean + SEM of multiple datapoints collected from three animals. Unfilled bars are used for vehicle (Veh) treated WT, filled bars are used for vehicle (Veh) treated Magel2-KO; blue is used for males of both genotypes and ages, red for females of both genotypes and ages. Data were analyzed by three-way ANOVA, followed by a Tukey’s multiple comparisons post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. When a comparison was approaching statistical significance, the corresponding p-value was reported on the appropriate graph. Datasets and detailed statistical analyses are reported in the Supplementary Table 1.