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. 2023 Mar 14;14:1141800. doi: 10.3389/fphar.2023.1141800

FIGURE 3.

FIGURE 3

Effect and toxicity of arctiin-encapsulated DSPE-PEG bubble-like nanoparticles (ARC@DPBNPs) on pulmonary fibrosis in the mouse model (A) Schematic representation of the regimens of ARC@DPBNPs, phosphate-buffered saline, and bleomycin local pulmonary administration in mice (B) Representative photographs of lung sections of the pulmonary fibrosis in a mouse model treated with various ARC@DPBNP concentrations stained with hematoxylin–eosin (HE) and Masson’s trichrome (MA). Scale bar = 50 μm. (C) Fibrotic scores were analyzed. (D) Representative results of senescence-associated ß-galactosidase staining of mice lung tissues. Scale bar = 50 μm. (E) Senescent marker p21 in mice lung tissues measured through Western blotting. (F) Hematoxylin and Eosin staining of the heart, liver, spleen, and kidney performed after intrapulmonary administration of ARC@DPBNPs (3 mg/kg/d) for 21 d *p < 0.05, **p < 0.01, ***p < 0.001.