Table 1.
Metrics of diversity estimates of the microbial community associated with different parts of the digestive tract (DT) of whitefish and cestodes parasitizing the intestine of C. l. pidschian.
| Fish/cestodes | Segment of DT | Type of sample | Number of analyzed samples | Number of ASV | Shannon | Simpson | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Phylum | Class | Order | Family | Genus | Total number | ||||||
| Infected “dwarf” C. l. pravdinellus | Stomach | Content | 8 | 11.8 ± 1.0 | 16.4 ± 1.6 | 36.8 ± 4.3 | 44.6 ± 5.6 | 41.1 ± 5.3 | 106.8 ± 16.8 | 2.7 ± 0.2 | 0.8 ± 0.0 |
| Mucosa | 12 | 8.8 ± 1.0 | 11.8 ± 1.5 | 23.3 ± 3.3 | 27.8 ± 4.4 | 25.7 ± 4.4 | 55.4 ± 10.2 | 2.3 ± 0.3 | 0.7 ± 0.1 | ||
| Washout | 13 | 10.9 ± 0.6 | 15.4 ± 1.0 | 34.0 ± 2.6 | 41.4 ± 3.6 | 42.6 ± 4.8 | 105.8 ± 20.6 | 3.3 ± 0.2 | 0.9 ± 0.0 | ||
| Mean ± SE | 10.4 ± 0.5 | 14.4 ± 0.8 | 30.9 ± 2.1 | 37.3 ± 2.8 | 36.3 ± 3.1 | 88.2 ± 10.6 | 2.8 ± 0.2 | 0.8 ± 0.0 | |||
| Intestine (anterior) | Content | 10 | 7.0 ± 1.0 | 9.7 ± 1.5 | 20.5 ± 3.5 | 23.3 ± 4.6 | 23.1 ± 5.3 | 57.5 ± 22.4 | 2.0 ± 0.3 | 0.7 ± 0.1 | |
| Mucosa | 10 | 5.5 ± 0.7 | 7.3 ± 0.8 | 14.0 ± 1.8 | 15.4 ± 2.0 | 16.0 ± 2.7 | 26.6 ± 4.3 | 1.3 ± 0.1 | 0.5 ± 0.1 | ||
| Washout | 9 | 5.4 ± 0.6 | 7.0 ± 0.7 | 16.7 ± 1.4 | 17.0 ± 1.6 | 17.1 ± 2.0 | 27.7 ± 3.2 | 2.7 ± 0.2 | 0.9 ± 0.0 | ||
| Mean ± SE | 6.0 ± 0.5 | 8.0 ± 0.6 | 17.1 ± 1.5 | 18.6 ± 1.9 | 18.8 ± 2.1 | 37.6 ± 8.1 | 2.0 ± 0.2 | 0.7 ± 0.0 | |||
| Intestine (posterior) | Content | 12 | 11.4 ± 0.8 | 16.0 ± 1.3 | 35.8 ± 3.0 | 43.1 ± 3.8 | 43.6 ± 5.0 | 144.8 ± 2.4 | 2.5 ± 0.3 | 0.7 ± 0.1 | |
| Mucosa | 11 | 6.1 ± 0.6 | 8.5 ± 0.7 | 17.0 ± 1.5 | 20.4 ± 2.1 | 23.0 ± 2.8 | 40.9 ± 4.3 | 2.5 ± 0.1 | 0.8 ± 0.1 | ||
| Washout | 13 | 8.2 ± 0.4 | 11.1 ± 0.6 | 24.8 ± 1.6 | 28.3 ± 2.0 | 30.6 ± 2.5 | 54.2 ± 3.2 | 3.4 ± 0.2 | 0.9 ± 0.0 | ||
| Mean ± SE | 8.6 ± 0.7 | 11.9 ± 1.2 | 26.1 ± 2.6 | 30.8 ± 3.4 | 32.6 ± 3.8 | 80.3 ± 12.9 | 2.8 ± 0.2 | 0.8 ± 0.0 | |||
| Uninfected “normal” C. l. pidschian | Stomach | Content | 12 | 20.3 ± 1.6 | 35.2 ± 3.7 | 70.3 ± 6.8 | 89.1 ± 8.2 | 100.6 ± 11.1 | 399.0 ± 62.7 | 3.2 ± 0.4 | 0.8 ± 0.1 |
| Mucosa | 12 | 8.9 ± 1.2 | 12.6 ± 2.4 | 24.3 ± 5.1 | 26.7 ± 5.7 | 26.5 ± 6.3 | 83.6 ± 25.5 | 2.8 ± 0.3 | 0.8 ± 0.0 | ||
| Washout | 12 | 12.8 ± 2.0 | 20.8 ± 3.4 | 42.7 ± 7.4 | 55.2 ± 9.5 | 58.1 ± 10.5 | 184.5 ± 43.0 | 3.3 ± 0.5 | 0.8 ± 0.1 | ||
| Mean ± SE | 14.0 ± 1.3 | 22.9 ± 2.5 | 45.9 ± 5.1 | 57.1 ± 6.6 | 61.9 ± 7.9 | 223.4 ± 34.6 | 3.1 ± 0.2 | 0.8 ± 0.0 | |||
| Intestine (anterior) | Content | 13 | 14.6 ± 1.4 | 23.6 ± 3.1 | 51.3 ± 6.1 | 66.4 ± 8.0 | 76.5 ± 10.8 | 312.2 ± 59.7 | 4.0 ± 0.4 | 0.9 ± 0.1 | |
| Mucosa | 10 | 7.4 ± 1.1 | 9.0 ± 1.1 | 19.3 ± 2.8 | 23.6 ± 3.4 | 24.5 ± 4.2 | 54.6 ± 8.3 | 2.5 ± 0.3 | 0.8 ± 0.0 | ||
| Washout | 10 | 6.2 ± 0.8 | 7.7 ± 1.0 | 14.6 ± 2.9 | 16.7 ± 4.0 | 16.0 ± 4.3 | 35.3 ± 10.9 | 1.5 ± 0.3 | 0.5 ± 0.1 | ||
| Mean ± SE | 9.9 ± 1.0 | 14.3 ± 1.9 | 30.3 ± 4.2 | 38.2 ± 5.6 | 42.2 ± 7.0 | 150.2 ± 32.8 | 2.8 ± 0.3 | 0.7 ± 0.0 | |||
| Intestine (posterior) | Content | 14 | 13.9 ± 1.2 | 22.3 ± 2.3 | 49.6 ± 5.2 | 63.1 ± 7.1 | 68.8 ± 8.5 | 262.2 ± 48.9 | 3.2 ± 0.4 | 0.8 ± 0.1 | |
| Mucosa | 10 | 6.3 ± 0.6 | 7.9 ± 0.8 | 12.9 ± 1.0 | 15.1 ± 0.9 | 14.1 ± 0.9 | 30.3 ± 4.5 | 1.8 ± 0.2 | 0.6 ± 0.1 | ||
| Washout | 9 | 6.8 ± 0.6 | 8.8 ± 0.8 | 17.1 ± 1.5 | 18.4 ± 2.1 | 16.8 ± 2.5 | 28.3 ± 3.8 | 1.9 ± 0.2 | 0.7 ± 0.1 | ||
| Mean ± SE | 9.7 ± 1.0 | 14.3 ± 1.7 | 29.8 ± 3.7 | 36.6 ± 4.9 | 38.3 ± 5.7 | 128.2 ± 28.8 | 2.4 ± 0.2 | 0.7 ± 0.0 | |||
| Infected “normal” C. l. pidschian | Stomach | Content | 8 | 20.7 ± 2.2 | 36.9 ± 5.2 | 74.3 ± 8.9 | 91.4 ± 9.9 | 99.5 ± 13.0 | 455.6 ± 108.2 | 3.6 ± 0.6 | 0.8 ± 0.1 |
| Mucosa | 8 | 11.4 ± 0.9 | 15.6 ± 1.4 | 28.5 ± 2.5 | 33.6 ± 3.5 | 32.7 ± 4.9 | 84.3 ± 17.3 | 3.7 ± 0.2 | 0.9 ± 0.0 | ||
| Washout | 7 | 12.9 ± 2.0 | 19.4 ± 3.9 | 37.3 ± 7.3 | 48.6 ± 9.2 | 51.2 ± 11.1 | 260.0 ± 71.8 | 3.7 ± 0.6 | 0.9 ± 0.1 | ||
| Mean ± SE | 15.1 ± 1.3 | 24.1 ± 2.8 | 47.0 ± 5.4 | 58.2 ± 6.5 | 61.5 ± 7.8 | 266.9 ± 53.4 | 3.7 ± 0.3 | 0.9 ± 0.0 | |||
| Intestine (anterior) | Content | 9 | 16.5 ± 0.9 | 27.5 ± 2.1 | 55.6 ± 4.9 | 71.1 ± 7.1 | 76.5 ± 8.6 | 370.8 ± 70.9 | 4.1 ± 0.6 | 0.9 ± 0.1 | |
| Mucosa | 9 | 8.2 ± 0.8 | 10.4 ± 1.1 | 19.2 ± 2.3 | 22.4 ± 2.9 | 20.4 ± 3.2 | 37.8 ± 7.1 | 2.3 ± 0.3 | 0.7 ± 0.1 | ||
| Washout | 4 | 6.6 ± 1.4 | 7.8 ± 1.5 | 11.6 ± 2.8 | 13.4 ± 3.9 | 11.6 ± 4.9 | 15.75 ± 4.3 | 1.87 ± 0.3 | 0.74 ± 0.1 | ||
| Mean ± SE | 11.3 ± 1.0 | 16.9 ± 2.0 | 32.6 ± 4.4 | 40.6 ± 5.9 | 41.6 ± 6.8 | 170.0 ± 46.1 | 2.9 ± 0.3 | 0.8 ± 0.0 | |||
| Intestine (posterior) | Content | 8 | 16.6 ± 2.0 | 29.8 ± 4.3 | 64.2 ± 8.8 | 80.0 ± 11.9 | 92.0 ± 16.4 | 602.6 ± 124.9 | 5.1 ± 0.3 | 0.9 ± 0.0 | |
| Mucosa | 4 | 9.6 ± 2.1 | 13.6 ± 4.2 | 25.7 ± 8.5 | 30.1 ± 10.6 | 31.7 ± 13.0 | 60.5 ± 38.6 | 2.0 ± 0.4 | 0.6 ± 0.1 | ||
| Washout | 6 | 7.7 ± 0.6 | 10.5 ± 0.9 | 21.4 ± 2.0 | 24.6 ± 2.3 | 25.2 ± 2.6 | 53.0 ± 15.8 | 2.2 ± 0.5 | 0.7 ± 0.1 | ||
| Mean ± SE | 11.7 ± 1.3 | 19.1 ± 2.8 | 38.3 ± 6.2 | 46.7 ± 8.0 | 51.0 ± 10.2 | 298.9 ± 85.3 | 3.4 ± 0.4 | 0.8 ± 0.1 | |||
| Cestodes | D0 | 7 | 11.4 ± 2.0 | 18.4 ± 4.2 | 37.7 ± 8.5 | 47.4 ± 11.0 | 52.3 ± 13.6 | 218.1 ± 91.6 | 2.8 ± 0.6 | 0.7 ± 0.1 | |
| D1 | 21 | 5.2 ± 0.4 | 6.6 ± 0.5 | 14.7 ± 1.1 | 19.2 ± 1.2 | 21.7 ± 1.5 | 38.7 ± 2.7 | 1.9 ± 0.2 | 0.7 ± 0.0 | ||
| D2 | 21 | 4.9 ± 0.5 | 6.4 ± 0.6 | 13.3 ± 1.2 | 17.0 ± 1.6 | 18.2 ± 2.0 | 34.2 ± 4.6 | 1.5 ± 0.2 | 0.6 ± 0.1 | ||
| D3 | 21 | 4.7 ± 0.2 | 6.0 ± 0.2 | 12.6 ± 0.7 | 15.5 ± 1.0 | 15.6 ± 1.2 | 29.1 ± 2.5 | 1.7 ± 0.2 | 0.6 ± 0.1 | ||
| D4 | 21 | 4.0 ± 0.3 | 5.2 ± 0.3 | 11.8 ± 0.8 | 14.7 ± 0.9 | 15.7 ± 1.2 | 27.9 ± 2.1 | 1.7 ± 0.2 | 0.6 ± 0.1 | ||
| D5 | 21 | 4.3 ± 0.2 | 5.7 ± 0.2 | 11.7 ± 0.6 | 14.6 ± 0.9 | 15.8 ± 1.2 | 28.6 ± 2.4 | 1.7 ± 0.2 | 0.7 ± 0.1 | ||
| Mean ± SE | 4.6 ± 0.2 | 6.0 ± 0.2 | 12.8 ± 0.4 | 16.2 ± 0.5 | 17.4 ± 0.7 | 31.7 ± 1.4 | 1.7 ± 0.1 | 0.6 ± 0.0 | |||
| D6 | 21 | 4.2 ± 0.2 | 5.6 ± 0.2 | 10.8 ± 0.7 | 13.9 ± 0.9 | 15.1 ± 1.1 | 26.5 ± 2.1 | 1.7 ± 0.2 | 0.6 ± 0.1 | ||
| D7 | 20 | 5.6 ± 0.2 | 7.4 ± 0.3 | 16.5 ± 0.8 | 22.5 ± 1.0 | 28.2 ± 1.4 | 51.1 ± 2.3 | 2.2 ± 0.2 | 0.7 ± 0.0 | ||
The bold character indicates mean and standard error.