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. 2023 Mar 27;14:1709. doi: 10.1038/s41467-023-37256-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Chromatin binding properties of AJSZ in human dermal fibroblasts (HDFs) have not been characterized. b Number of binding sites determined by ChIP-seq for each factor. Peaks were merged from two samples. c Percentage of AJSZ binding sites located in open or closed chromatin. scATAC-seq was used to determine the state (open or closed) of the chromatin. d Top enriched motif in open chromatin bound by AJZ. e Co-occupancy analysis for AJZ at regions of open chromatin. f AJZ binding distribution at annotated regulatory regions in open chromatin. g Top enriched motifs in closed chromatin bound by AJS. h siStat4, 5a, or 6 respectively increase reprogramming efficiency as compared to siControl in iMGT-MEF assay. n = 8 biologically independent experiments for siControl and siStat4, 5a or 6 treated. Student’s t-test: **p < 0.01. Groups were compared using two-tailed unpaired. Data were presented as mean values ± standard deviation. i Representative images for siStat4 and siControl-transfected iMGT-MEFs 3 days post-Dox(MGT) treatment. Scale bars: 50 µm. j Motif analysis at regions of AJS-bound closed chromatin show enrichment for reprogramming TF motifs (i.e., MEF2C, ASCL1, and KLF4). k Schematic summarizing AJSZ binding properties at regions of open and closed chromatin in HDFs.