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. 2023 Mar 14;14:1134653. doi: 10.3389/fpls.2023.1134653

Figure 4.

Figure 4

Plant immune responses in the loss-of-function mutants of OsHPP04 after flg22 treatment. (A) ROS burst from the different shoot discs treated with 1 μM flg22 was measured by Photek camera system between 0 and 30 min. Bars represent mean ± SE (n = 8 biological replicates). (B) Callose deposition in rice roots after treatment with 1μM flg22 for 12 h. The average amount of callose depositions were measured by ImageJ. Data were analyzed by a two-tailed Student’s t-test (**P < 0.01. Values are mean ± SE). Bars = 50 μm. (C) Expression levels of defence-related genes (OsKS4, OsPAL4, OsEDS1, OsPR1a and OsPR4) were determined by quantitative real-time polymerase chain reaction in rice after treatment with 1 μM flg22 for 1 h. Values are mean ± SE, *P<0.05, Student’s t-test. The rice Ubiquitin gene (OsUBQ) was used as an internal control. WT: wild-type rice; TF-H4-1 and TF-H6-1: two ‘transgene-free’ OsHPP04 mutant lines. Three independent experiments were performed with similar results.