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The major agronomic traits of ‘transgene-free’ OsHPP04 knockout rice at mature stage. (A) The morphological phenotypes of rice. (B) Panicle and seeds. (C) The grain number per panicle, seed setting rate and thousand-grain weight. Data were analyzed by a two-tailed Student’s t-test (ns P > 0.05. Values are mean ± SE). WT: wild-type rice. TF-H4-1 and TF-H6-1: two ‘transgene-free’ OsHPP04 mutant lines. Bars = 5 cm.
