Table 1.

Primers used to construct MPI-N, MPI-C, and MPI-SP expression plasmids.

Primer name	Sequence (5’-3’)
MPI-N-sense	TATGATCGACTGGAAAAAACTGCTGGACGCTGCGAAACAAATCCTGGGTGGTGGTG
MPI-N-antisense	CTAGTACCACCACCCAGGATTTGTTTCGCAGCGTCCAGCAGTTTTTTCCAGTCGATTA
MPI-C-sense	GATCCATCGAAGGTCGTATTGACTGGAAAAAACTGCTGGATGCTGCTAAGCAGATTCTCTAAC
MPI-C-antisense	TCGAGTTAGAGAATCTGCTTAGCAGCATCCAGCAGTTTTTTCCAGTCAATACGACCTTCGATG
SP-Forward-1	GGGTTTCATATGAAAAAGACAGCTATCGCGATTGCAGTGGCACTGGCTGGTTTCGCTAC
SP-Forward-2	GGCTGGTTTCGCTACCGTAGCGCAGGCCGGTGGTGGTACTAGTATCGACTGGAAAAAAC
SP-Reverse	CCGCTCGAGCTGCAGGTAC

DNA sequences with bold indicate MPI encoding sequences. DNA sequences with underline indicate pairing sequence for the second PCR.