Figure 2.

- Levels of B-lymphocytes and their subsets in T2DM patients and healthy individuals. Lymphocytes were isolated from the peripheral blood of the T2DM patients and healthy individuals. The cells were then stained with anti-CD19 (FITC), CD27 (PerCyp Cy5.5), and CD38 (APC) and analyzed using flow cytometry. A) Representative dot plots showing the percentage of CD19+ B-cells in the samples obtained from healthy individuals and T2DM patients. B) The percentage of CD19+ cells in both groups is presented. C) Representative dot plots showing the percentage of B-cell subsets in the samples obtained from healthy individuals and T2DM according to CD27 and CD38 expression. D) The percentage of CD27-/CD38+ cells in both groups is presented. E) The percentage of CD27+/CD38- cells in both groups is presented. Data were analyzed using an unpaired t-test. Values are presented as mean±SD. ^*** p≤0.001, ^**** p≤0.0001, T2DM: type 2 diabetes mellitus