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. Author manuscript; available in PMC: 2023 Mar 28.
Published in final edited form as: Matrix Biol. 2022 Sep 13;113:1–21. doi: 10.1016/j.matbio.2022.09.001

Fig. 3.

Fig. 3.

Collagen VI increases matrix adhesiveness

T cells adhesion to extracellular matrix (ECM) coated plates was tested. (A) The adhesiveness of T cells was enhanced with increasing concentrations of collagen VI. Phorbol myristate acetate (PMA) pre-treated T cells showed a higher adherence compared to untreated T cells. (B) The increased adhesiveness of T cells was not only observed in in collagen VI coated plates but also in collagen I and collagen IV coated plates and adhesion increased in all groups after exposure of T cells to PMA (n = 4~6, t test). (C) Human intestinal myofibroblasts (HIMFs) were transfected with small interfering (si)RNA targeting COL6A1 prior to generation of 2D ECM scaffolds. T cell adhesion was robustly decreased in the extracellular matrix (ECM) derived from HIMFs which were transfected by COL6A1 siRNA compared to scrambled siRNA (n = 6, t test). *, p < 0.05, **, p < 0.01, ***, p < 0.001.