Figure 4.

(A) Images of a representative sample of the live Caco-2 cell line incubated with DCM-NH-Pro-Gly (2.5 μM) recorded in the red (left, λ_ex = 450 nm, λ_em = 648–722 nm) and green (middle, λ_ex = 450 nm, λ_em = 533–557 nm) channels at six different time points. Ratio R/G images (right) obtained at the same times. (B) Representation of the average values of the ratio R/G from five independent experiments. Error bars represent SE. (C) Double-logarithmic representation of the kinetics. Error bars represent SE. The red line is a linear fit with an intercept of 0.964 ± 0.004 and a slope of 0.215 ± 0.003 (R² = 0.995).