Fig. 5.

In vitro bacterial GUS activity toward testosterone glucuronide deconjugation. GUS activity toward testosterone glucuronide in fecal samples at 1 µM TG (A). Deconjugation of TG to testosterone by GUS enzymes from various gut bacterial species at 20 µM TG concentration (B). TG disappearance with time in the presence of GUS enzymes from E. coli (C) and E. eligens (D) at 10 µM TG concentration. The experiments were performed in triplicates, where percent coefficient of variation (%CV) was within 30% as indicated in (C) and (D). TG disappearance rate [0 minutes versus individual timepoints; (C) and (D)] was compared using ANOVA followed by Dunnett’s T3 multiple comparisons test, with *P < 0.05; **P < 0.01; ***P < 0.001.