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. Author manuscript; available in PMC: 2024 Mar 8.
Published in final edited form as: Nano Lett. 2023 Feb 20;23(5):1711–1716. doi: 10.1021/acs.nanolett.2c04379

Figure 2.

Figure 2.

pSMdM quantification of intra-ER diffusion and charge effects. (a,b) Representative pSMdM super-resolution D maps for ER-Dendra2 in the ER lumen of live COS-7 cells, after Brefeldin A treatment (a) or ATP depletion (b), respectively. (c) pSMdM-determined D values of ER-Dendra2 in untreated (Org), Brefeldin A-treated (BA), and ATP-depleted (-ATP) COS-7 cells. (d) pSMdM-determined D values for the different charge-varied versions of ER-Dendra2 diffusing in the ER lumen of live COS-7 cells. In (c,d), each circle presents the holistic D value extracted from an individual cell. Squares + bars: averages and standard deviations. (e) Representative pSMdM super-resolution D maps of the differently charged ER-Dendra2 versions. Note that different color scales are used in (a,b,e) to present the different D ranges.