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. Author manuscript; available in PMC: 2024 Mar 8.
Published in final edited form as: Nano Lett. 2023 Feb 20;23(5):1711–1716. doi: 10.1021/acs.nanolett.2c04379

Figure 4.

Figure 4.

Implications for a positively charged endogenous ER protein. (a) Surface charge models for the +6-charged wild-type (top) and 0-charged mutant (bottom) PPIB, generated using PyMOL (Version 2.0; Schrödinger, Inc.) based on the PPIB structure (PDB ID: 3ICH). Red and blue indicate negative and positive charges, respectively. (b,c) Representative pSMdM super-resolution D maps for the two proteins diffusing in the ER lumen of live COS-7 cells. Both proteins are tagged with the 0-charged version of Dendra2 FP. (d) Statistics of pSMdM-determined D of the two proteins in COS-7 cells. Circles: holistic D values of individual cells. Squares + bars: averages and standard deviations.