Fig. 4: Overall relationships among dorsal and ventral neuronal populations in human and mouse lumbar spinal cord.

A, Distributions of percell Silhouette scores in human and mouse spinal cord neurons, separated into dorsal and ventral groups. Higher silhouette scores indicate that cells belong to more clearly separated clusters. Cluster-level silhouette scores are shown in Supplemental Fig. S11. Two-way ANOVA, followed by Mann-Whitney tests for human and mouse dorsal vs ventral distributions were p < 0.0001, ****. B-C, UMAP of human neurons (B) and mouse neurons (C) colored by Silhouette score. D, Median gene expression correlation (Pearson’s R) of each cluster to other clusters, using the top 2,000 highly variable genes. Two-way ANOVA, followed by Mann-Whitney tests for human and mouse dorsal vs ventral distributions were p < 0.0001, ****. E-F, Heatmap of pairwise gene expression correlations (Pearson’s R) of human spinal cord clusters (E) and mouse spinal cord clusters (F, Russ et al.¹⁹). Correlation is colored from purple (low) to yellow (high). See also Supplemental Figure S14.