Table 2.
Primer sequences for target genes used in current study by means of real time qPCR.
| Target Gene | Primer Sequences (5′ → 3′) | Product Length | Annealing Temperature (°C) | GenBank Accession Number |
|---|---|---|---|---|
| TNF-α | F: GGGAGTGTGAGGGGTATCCT R: CTGCACCTTCTGTCTCGGTT |
93 | 60 | MH180383.1 |
| IL-1β | F: ACAAGCCGAACAAAGCACAC R: CTCCACATCTGGCTCACGTT |
106 | 61 | KY038171.1 |
| IL-4 | F: GCAGCTGATCCGATTCCTGA R: TCCAACGTACTCTGGTTGGC |
99 | 60 | NM_000589.4 |
| IL-6 | F: GGAGTGGCCAAGAACCAAGA R: ATGCTAAGGCACAGCACACT |
109 | 60 | AB028635.1 |
| IL-10 | F: ACCGTGATTGGCAGGTACAG R: CTGGCCCTGGGTTCACTTAG |
123 | 58 | JQ687536.1 |
| IL-12 | F: ATCCACTGGACCTCAGACCA R: CTCAGAGTCTCGCCTCCTCT |
122 | 59 | S82489.1 |
| INF-Y | F: TCCCAGAAGCTATCTGAGCAT R: CCACCGTCAGCTACATCTGAAT |
200 | 58 | NM_205149.2 |
| sIgA | F: TTCCTGAGTTGCCGAGTGAC R: AGGGATTTCTTGCTGGGAGC |
106 | 60 | DL232588.1 |
| MUC-2 | F: CGGTGATGACAACGACTCCA R: AAGTTTGCACAGTCGTTCGC |
163 | 60 | AF167707.1 |
| β-actin | F: CCTTCCTGGGTAGGTGTCG R: TGGCGTAGAGGTCCTTCCTG |
188 | 60 | AJ312193.1 |
TNF-α: Tumor necrosis factor-alpha; IL: Interleukin; INF-Y: Interferon-gamma; sIgA: Secretory Immunoglobulin A; MUC-2: Secretory mucin 2. Design and test primers for this sequence using Primer-BLAST.