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His-tag–based pull-down assay of the SqrR–TrxC interaction. SqrR-FLAG was mixed with Ni-resin in the absence (−TrxC) and presence (+TrxC) of His-tagged TrxC. Input and output were analyzed by Western blotting using an anti-FLAG antibody to detect SqrR-FLAG. The numbers to the left of the images indicate molecular weight based on size markers (kDa).
