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DUOX1 deficiency in SKCM promotes metastasis. (A) Schematic diagram of adult allotransplantation assays. (B) Representative images of the progression of metastasis (arrows). (C) Metastasis-free curve of adult zebrafish transplanted with MinicoopR mitfa:NRAS-Q61R/mitfa:DN-DUOX1 and MinicoopR mitfa:NRAS-Q61R/mitfa:EGFP (control). ** p < 0.01 according to a Log rank Mantel–Cox test. DN-DUOX1: n = 5 tumors and 111 recipient fish; EGFP: n = 3 tumors and 72 recipient fish.
