Table 1.
Effect of the source of explants and number of subcultures on plantlets height (PH), number of nodes (NN), number of shoots (NS), and canopy surface area (CSA) during in vitro multiplication of cannabis.
| Subculture | Explant’s Source | PH ± SE (mm) | NN ± SE | NS ± SE | CSA ± SE (mm2) |
|---|---|---|---|---|---|
| I | A | 43.45 ± 1.113 | 8.5 ± 0.501 | 1 ± 0.000 | 994 ± 31.093 |
| I | B | 45.335 ± 2.192 | 8.5 ± 0.501 | 1 ± 0.000 | 1004 ± 5.015 |
| I | C | 43.88 ± 2.648 | 8.5 ± 1.504 | 1 ± 0.000 | 1296 ± 60.179 |
| I | D | 38.25 ± 2.848 | 7.5 ± 0.501 | 1 ± 0.000 | 1119.5 ± 45.636 |
| II | A | 49.71 ± 1.525 | 8.5 ± 0.501 | 1 ± 0.000 | 1927.3 ± 74.221 |
| II | B | 43.995 ± 2.533 | 8.5 ± 1.504 | 1.5 ± 0.501 | 2293.5 ± 57.672 |
| II | C | 41.21 ± 2.046 | 7.5 ± 0.501 | 1 ± 0.000 | 1392 ± 67.200 |
| II | D | 35.475 ± 2.071 | 6 ± 1.003 | 1 ± 0.000 | 1025 ± 129.385 |
| III | A | 39.33 ± 0.923 | 7.5 ± 0.501 | 1 ± 0.000 | 1124.5 ± 58.675 |
| III | B | 41.005 ± 1.439 | 8 ± 1.003 | 1.5 ± 0.501 | 1388 ± 19.057 |
| III | C | 30.36 ± 1.033 | 5 ± 1.003 | 1 ± 0.000 | 889.5 ± 15.546 |
| III | D | 29.22 ± 0.802 | 3.5 ± 0.501 | 1 ± 0.000 | 803 ± 22.066 |
| IV | A | 36.865 ± 2.553 | 7 ± 1.003 | 1 ± 0.000 | 1182.5 ± 46.639 |
| IV | B | 36.54 ± 1.113 | 6.5 ± 1.504 | 1 ± 0.000 | 968.5 ± 37.612 |
| IV | C | 33.075 ± 1.449 | 4 ± 1.003 | 1 ± 0.000 | 957 ± 21.063 |
| IV | D | 28.995 ± 0.707 | 3.5 ± 0.501 | 1 ± 0.000 | 967.5 ± 46.639 |
| p value | |||||
| Explant | <0.0001 | 0.0010 | 0.5847 | <0.0001 | |
| Subculture | <0.0001 | 0.0014 | 0.1546 | <0.0001 | |
| Explant × subculture | 0.1400 | 0.6394 | 0.7271 | <0.0001 | |
A: Explant derived from basal part; B: explant derived from near-basal part; C: explant derived from middle part; D: explant derived from apical part; SE: standard error.