Figure 2.

Survival and recovery parameters after CA-CPR. (A) Kaplan-Meier plot for mouse survival after successful resuscitation following 8 min of cardiac arrest (CA) within the 28-day observation period. No significant differences between the different groups (controls=Ctrl. (blue): n = 37; Pep19-2.5-treated (green): n = 34; and Pep19-4LF-treated (berry): n = 37) were detected in survival at the end of observation (p = 0.465). At 16 h of observation, the difference between the groups was greatest and between control and Pep19-2.5-treated with p = 0.029 significant. (B) Course of body weight in the observation period of 28 days of all groups was presented as mean ± SEM. Tendentially, the body weight of the control mice does not decrease as much as in the treated groups on Day 2 post-CA-CPR. Thereafter, the animals of all groups gain weight continuously. There was no significant difference between the groups over the time period. Only on Day 28 did the Pep19-4LF treated animals (n = 5) show a higher body weight compared to the control animals (n = 8; p = 0.040). (C) The nesting behavior (mean ± SEM; n number of animals) was not significantly different between the investigated groups on different days as well as the time (days) to reach the initial nesting score after CA-CPR. The difference between control and Pep19-4LF groups was just not significant on Day 5 (p = 0.056). On Days 7 and 8, statistical comparison between groups was not performed because only 1 control animal and 2 animals per peptide treatment group were left in the experiment. For the animals that had reached the nesting score before resuscitation, the experiment ended and so did the single animal housing. Schemes follow the same formatting.