Figure 5.

Mt3 overexpression reduces adipogenesis-related gene expression in 3T3−L1 cells. 3T3−L1 cells were transfected with HA−MT3 plasmid (0.25, 0.5 μg) prior to contact inhibition and induced adipocyte differentiation using differentiation cocktail. (a–g) The mRNA levels of Mt3, Pparg, Cebpa, Cebpb, Adiponectin, Fabp4, Fasn, and Glut4 in the different groups were compared by RT-qPCR. β-actin was used as a loading control. The data are presented as the means ± SEM. ** p < 0.01, *** p < 0.001 were calculated by one-way ANOVA followed by Tukey’s Test.