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. 2023 Mar 4;12(3):640. doi: 10.3390/antiox12030640

Figure 6.

Figure 6

MT3 indirectly suppresses PPARγ transcriptional activities. (a,b) HEK 293T cells were transfected with indicated HA−MT3 (0.5 μg) or combinations of HA−PPARγ (0.125 μg) and HA-MT3 (0.5 μg) along with aP2−Luc or PPRE-Luc reporter. pCMV−β−gal (0.025 μg) was used to normalize the transfection efficiency. HEK 293T cells were incubated with 0.5 μM rosiglitazone and DMSO 24 h after transfection and cultured for an additional 24 h. The luciferase reporter activities were measured following 48 h transfection. The data are presented as the means ± SEM. ** p < 0.01, *** p < 0.001 were calculated by one-way ANOVA followed by Tukey’s Test. (c,d) HEK 293T cells were transfected with indicated expression plasmids (HA−MT3, Myc−PPARγ, or empty Myc vector) for immunoprecipitation.