Table 1.
List of differentially expressed proteins in the proteome of differentiated 3T3-L1 cells after Pcyox1 silencing with respect to control cells, treated with a negative construct.
| Accession | Unique Peptides | q Value | Max Fold Change | Highest Mean Condition |
Description |
|---|---|---|---|---|---|
| Reduced in Pcyox1 silenced cells | |||||
| P54310 | 3 | 0.003 | 7.07 | NEG | Hormone-sensitive lipase OS = Mus musculus GN = Lipe |
| Q9CQF9 | 2 | 0.001 | 4.84 | NEG | Prenylcysteine oxidase OS = Mus musculus GN = Pcyox1 |
| P16015 | 5 | 0.001 | 4.73 | NEG | Carbonic anhydrase 3 OS = Mus musculus GN = Car3 |
| P04117 | 5 | 0.003 | 3.51 | NEG | Fatty acid-binding protein_ adipocyte OS = Mus musculus GN = Fabp4 |
| Q8CGN5 | 5 | 0.001 | 3.34 | NEG | Perilipin-1 OS = Mus musculus GN = Plin1 |
| Q8K3K7 | 3 | 0.003 | 2.91 | NEG | 1-acyl-sn-glycerol-3-phosphate acyltransferase beta OS = Mus musculus GN = Agpat2 |
| Q91WU0 | 2 | 0.006 | 2.77 | NEG | Carboxylesterase 1F OS = Mus musculus GN = Ces1f |
| Q08857 | 2 | 0.012 | 2.32 | NEG | Platelet glycoprotein 4 OS = Mus musculus GN = Cd36 |
| P13707 | 9 | 0.006 | 2.32 | NEG | Glycerol-3-phosphate dehydrogenase [NAD(+)]_ cytoplasmic OS = Mus musculus GN = Gpd1 |
| P28271 | 3 | 0.004 | 2.18 | NEG | Cytoplasmic aconitate hydratase OS = Mus musculus GN = Aco1 |
| P16125 | 2 | 0.011 | 2.03 | NEG | L-lactate dehydrogenase B chain OS = Mus musculus GN = Ldhb P |
| P24270 | 2 | 0.004 | 1.92 | NEG | Catalase OS = Mus musculus GN = Cat |
| Q63918 | 4 | 0.001 | 1.92 | NEG | Serum deprivation-response protein OS = Mus musculus GN = Sdpr |
| Q05920 | 18 | 0.001 | 1.88 | NEG | Pyruvate carboxylase_ mitochondrial OS = Mus musculus GN = Pc |
| P31786 | 2 | 0.021 | 1.87 | NEG | Acyl-CoA-binding protein OS = Mus musculus GN = Dbi |
| O88492 | 3 | 0.019 | 1.86 | NEG | Perilipin-4 OS = Mus musculus GN = Plin4 |
| P45952 | 3 | 0.006 | 1.81 | NEG | Medium-chain specific acyl-CoA dehydrogenase_ mitochondrial OS = Mus musculus GN = Acadm |
| Q8BMS1 | 13 | 0.007 | 1.80 | NEG | Trifunctional enzyme subunit alpha_ mitochondrial OS = Mus musculus GN = Hadha |
| P97807 | 6 | 0.004 | 1.79 | NEG | Fumarate hydratase_ mitochondrial OS = Mus musculus GN = Fh |
| Q8BH95 | 3 | 0.037 | 1.70 | NEG | Enoyl-CoA hydratase_ mitochondrial OS = Mus musculus GN = Echs1 |
| Q9WTP7 | 2 | 0.007 | 1.68 | NEG | GTP:AMP phosphotransferase AK3_ mitochondrial OS = Mus musculus GN = Ak3 |
| P49817 | 2 | 0.016 | 1.53 | NEG | Caveolin-1 OS = Mus musculus GN = Cav1 |
| Q9CZU6 | 4 | 0.017 | 1.51 | NEG | Citrate synthase_ mitochondrial OS = Mus musculus GN = Cs |
| P62897 | 2 | 0.005 | 1.49 | NEG | Cytochrome c_ somatic OS = Mus musculus GN = Cycs |
| Q9EQ20 | 3 | 0.001 | 1.48 | NEG | Methylmalonate-semialdehyde dehydrogenase [acylating]_ mitochondrial OS = Mus musculus GN = Aldh6a1 |
| Q9JHI5 | 2 | 0.007 | 1.48 | NEG | Isovaleryl-CoA dehydrogenase_ mitochondrial OS = Mus musculus GN = Ivd |
| Q9CZ13 | 3 | 0.009 | 1.47 | NEG | Cytochrome b-c1 complex subunit 1_ mitochondrial OS = Mus musculus GN = Uqcrc1 |
| O54724 | 6 | 0.001 | 1.47 | NEG | Polymerase I and transcript release factor OS = Mus musculus GN = Ptrf |
| Q8BH64 | 8 | 0.003 | 1.46 | NEG | EH domain-containing protein 2 OS = Mus musculus GN = Ehd2 |
| P17751 | 9 | 0.001 | 1.46 | NEG | Triosephosphate isomerase OS = Mus musculus GN = Tpi1 |
| Q9D3D9 | 2 | 0.010 | 1.46 | NEG | ATP synthase subunit delta_ mitochondrial OS = Mus musculus GN = Atp5d |
| Q8BMF4 | 5 | 0.011 | 1.45 | NEG | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex_ mitochondrial OS = Mus musculus GN = Dlat |
| Q60597 | 4 | 0.022 | 1.44 | NEG | 2-oxoglutarate dehydrogenase_ mitochondrial OS = Mus musculus GN = Ogdh |
| Q9DB77 | 7 | 0.014 | 1.43 | NEG | Cytochrome b-c1 complex subunit 2_ mitochondrial OS = Mus musculus GN = Uqcrc2 |
| Q99L13 | 3 | 0.003 | 1.42 | NEG | 3-hydroxyisobutyrate dehydrogenase_ mitochondrial OS = Mus musculus GN = Hibadh |
| P08249 | 13 | 0.019 | 1.42 | NEG | Malate dehydrogenase_ mitochondrial OS = Mus musculus GN = Mdh2 |
| Q9DCW4 | 4 | 0.017 | 1.42 | NEG | Electron transfer flavoprotein subunit beta OS = Mus musculus GN = Etfb |
| Q99LC5 | 9 | 0.011 | 1.42 | NEG | Electron transfer flavoprotein subunit alpha_ mitochondrial OS = Mus musculus GN = Etfa |
| Q9CR62 | 2 | 0.003 | 1.41 | NEG | Mitochondrial 2-oxoglutarate/malate carrier protein OS = Mus musculus GN = Slc25a11 |
| P00405 | 4 | 0.046 | 1.41 | NEG | Cytochrome c oxidase subunit 2 OS = Mus musculus GN = Mtco2 |
| Q922Q1 | 2 | 0.034 | 1.41 | NEG | Mitochondrial amidoxime reducing component 2 OS = Mus musculus GN = Marc2 |
| P47738 | 8 | 0.016 | 1.40 | NEG | Aldehyde dehydrogenase_ mitochondrial OS = Mus musculus GN = Aldh2 |
| Q9D9V3 | 4 | 0.022 | 1.40 | NEG | Ethylmalonyl-CoA decarboxylase OS = Mus musculus GN = Echdc1 |
| Increased in Pcyox1 silenced cells | |||||
| Q60847 | 4 | 0.001 | 1.99 | shPCYOX1 | Collagen alpha-1(XII) chain OS = Mus musculus GN = Col12a1 |
| P37889 | 16 | 0.004 | 1.42 | shPCYOX1 | Fibulin-2 OS = Mus musculus GN = Fbln2 |