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. 2023 Mar 20;12(3):757. doi: 10.3390/antiox12030757

Figure 5.

Figure 5

Nanoceria reduces UVB-induced cell loss in primary human keratinocytes from healthy donors. Keratinocytes (KC) from healthy donors (5977, 5993, 5962) were exposed to UVB ± nanoceria (CNPs) for 15 s and let recover for 7–9 days. (A) Kinetics of viable (trypan blue-excluding) cell number expressed as fold over day 0. Statistical significance was calculated via Student’s t-test. * p < 0.05, with respect to irradiated cells. (B) Nanoceria (CNPs) reduce UV-induced keratinocyte cytostasis. The fraction of binucleated cells at 48 h after UVB exposure in the presence of the cytokinase inhibitor cytochalasin B is shown. Statistical significance was calculated via Student’s t-test. * p < 0.05, ** p < 0.01 and *** p < 0.001. (C) Phase-contrast images of UVB-irradiated cells ± nanoceria (CNPs) at the indicated time points. A * indicates DNA damage-induced enlarged cells. (D) Time course of apoptotic cells after UVB exposure ± nanoceria (CNPs). Statistical significance was calculated via Student’s t-test. * p < 0.05 with respect to irradiated cells.