. 2023 Feb 22;12(3):550. doi: 10.3390/antiox12030550

Table 6.

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activity (%) compared to control samples (without extract) of hydromethanolic and aqueous extracts of W. somnifera commercial samples.

Cholinesterase Inhibition
	AChE		BChE
Sample No.	Hydromethanolic Extracts	Aqueous Extracts	Hydromethanolic Extracts	Aqueous Extracts
1	83.24 ± 2.63 ^d*†	71.25 ± 0.59 ^e*	54.30 ± 1.76 ^bc*	101.35 ± 0.85 ^c*
2	64.36 ± 5.27 ⁱ*	46.46 ± 2.33 ^k*	53.60 ± 1.37 ^c*	90.17 ± 3.94 ^f*
3	63.83 ± 0.75 ⁱ*	53.33 ± 5.3 ⁱ*	40.07 ± 2.74 ^e*	94.29 ± 4.53 ^e*
4	40.69 ± 9.4 ^k*	59.17 ± 2.37 ^g*	21.24 ± 0.83 ^h*	60.30 ± 2.07 ^l*
5	123 ± 11.77 ^a*	46.46 ± 5.6 ^k*	36.79 ± 1.89 ^f*	76.44 ± 3.54 ^g*
6	84.04 ± 8.27 ^d*	57.29 ± 3.86 ^h*	55.96 ± 3.42 ^b*	105.71 ± 4.39 ^b*
7	107.45 ± 5.34 ^b	106.88 ± 5.04 ^b	78.28 ± 2.15 ^a*	138.27 ± 1.12 ^a*
8	21.28 ± 1.04 ^l*	67.29 ± 3.26 ^f*	18.55 ± 4.15 ⁱ*	69.94 ± 4.85 ⁱ*
9	77.93 ± 0.38 ^e*	109.58 ± 3.54 ^a*	21.41 ± 1.42 ^h*	75.68 ± 1.31 ^gh*
10	47.07 ± 0.58 ^j*	41.88 ± 2.06 ⁿ*	16.30 ± 1.17 ^j*	49.95 ± 3.74 ⁿ*
11	65.96 ± 1.50 ⁱ*	43.96 ± 2.06 ^l*	35.64 ± 0.21 ^f*	74.91 ± 5.05 ^h*
12	75.00 ± 4.51 ^f*	51.46 ± 2.67 ^j*	41.07 ± 0.75 ^e*	69.43 ± 1.04 ⁱ*
13	68.88 ± 1.88 ^h*	58.96 ± 1.31 ^g*	17.82 ± 2.83 ^ij*	33.53 ± 0.85 ^o*
14	71.81 ± 0.75 ^g*	36.46 ± 3.26 ^o*	46.42 ± 1.17 ^d*	99.35 ± 1.31 ^d*
15	83.24 ± 0.38 ^d*	42.92 ± 2.43 ^m*	46.15 ± 0.78 ^d*	58.07 ± 3.91 ^m*
16	89.89 ± 3.01 ^c	88.13 ± 4.45 ^c	27.11 ± 2.39 ^g*	61.64 ± 9.51 ^k*
17	120.74 ± 1.53 ^a*	106.46 ± 2.45 ^b*	47.81 ± 1.86 ^d*	89.19 ± 1.51 ^f*
18	87.50 ± 0.38 ^c*	77.5 ± 4.73 ^d*	53.64 ± 3.96 ^c*	64.42 ± 1.38 ^j*

^† Means followed by the same letter within the same column and for the same extraction method indicate no significant difference among samples according to Tukey’s HSD test (p < 0.05). The asterisk (*) symbol indicates significant differences between the different extraction protocols for the same sample according to Student’s t test (p < 0.05).