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. 2023 Mar 8;11(3):825. doi: 10.3390/biomedicines11030825

Figure 3.

Figure 3

HIF-MSCs affect the immune balance of mice with colitis via macrophages. (A). HIF-MSCs increased the expression of anti-inflammatory factors TGF-β and IL-10 and decreased that of pro-inflammatory factors IL-12β and IL-17A in the serum (**** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05). (B). HIF-MSC transplantation had a more noticeable effect on inflammatory cytokine expression in the colon tissue than MSC transplantation (**** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05). (C). CL2MDP liposomes were intravenously injected 48 h before and after HIF-MSC transplantation (CL2MDP-HIF group). Single CL2MDP treatment with colitis mice was the CLP group. Immunofluorescence detected F4/80 expression with CL2MDP treatment; the expression in the CLP group was significantly lower than in the TNBS group, indicating effective intestinal macrophage deletion (** p < 0.01). (D). DAI and pathological performance were worsened in the CL2MDP-HIF group compared with indicators due to a single HIF-MSC treatment (**** p < 0.0001, *** p < 0.001, * p < 0.05). (E). CL2MDP attenuated the effect of HIF-MSCs on inflammatory factors (**** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05). “ns” represented no significant difference.