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. 2023 Mar 14;14:1113194. doi: 10.3389/fimmu.2023.1113194

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Copyright © 2023 Oluka, Namubiru, Kato, Ankunda, Gombe, Cotten, The COVID-19 Immunoprofiling Team, Musenero, Kaleebu, Fox and Serwanga

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of In-House ELISA parallelism and accuracy. (A) shows the parallelism of OD450 values from assays using serum and plasma of the same test subject. (B) shows the accuracy of the in-house ELISA verified against negative and positive specimens in WHO anti-SARS-CoV-2 verification standard panels. The ROC-derived cut-off of 0.432 accurately categorised the standard negative and positive specimens.