Table 3.
ELISA “limits of detection” (LOD) and “limits of quantitation” (LOQ) using direct and indirect capture.
| Direct detection Variable Antigen-specific | LOD | LOQ |
|---|---|---|
| S-IgG | 0.310 | 0.940 |
| S-IgM | 0.383 | 1.160 |
| S-IgA | 0.217 | 0.658 |
| N-IgG | 0.213 | 0.647 |
| N-IgM | 0.089 | 0.271 |
| N-IgA | 0.215 | 0.651 |
| RBD-IgG | 0.121 | 0.366 |
| RBD-IgM | 0.238 | 0.720 |
| RBD-IgA | 0.106 | 0.321 |
| Indirect detection Variable Kappa and Lambda |
LOD | LOQ |
| spike: IgG | 0.104 | 0.315 |
| spike: IgM | 0.168 | 0.509 |
| spike: IgA | 0.196 | 0.594 |
| nucleoprotein: IgG Mean OD | 0.105 | 0.317 |
| nucleoprotein: IgM Mean OD | 0.092 | 0.279 |
| nucleoprotein: IgA Mean OD | 0.182 | 0.550 |
| RBD: IgG Mean OD | 0.202 | 0.612 |
| RBD: IgM Mean OD | 0.344 | 1.043 |
| RBD: IgA Mean OD | 0.302 | 0.914 |