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H2S synthesis in EO771 cells; effect of CBS/CSE or 3-MST inhibition. The H2S-synthesizing capacity of the cells was analyzed by live cell imaging. Incubation with the CBS/CSE inhibitor AOAA (A,C) or the 3-MST inhibitor HMPSNE (B,D) (24 h treatment of the cells) markedly reduced the H2S signal. N = 3 independent experiments ± SEM; *** p < 0.001 shows a significant inhibitory effect of AOAA or HMPSNE on the cellular H2S signal.
