Figure 9.

Effect of HMPSNE on the response to oxidative stress in EO771 cells. The 3-MST inhibitor (300 µM) did have a marked overall effect on oxidative stress induced cell death/loss of cell viability measured by LDH release (A,B) or MTT conversion (C,D), as assessed at 2 or 24 h. Nevertheless, at 2 h, in the presence of at 60 and 1000 µM H₂O₂, HMPSNE tended to exacerbate the degree of cell dysfunction, assessed by the suppression of MTT converting-ability (# p < 0.05 represents significant difference in MTT or LDH at the same concentration of H₂O₂, in the presence vs. absence of 300 µM HMPSNE). N = 3 independent experiments ± SEM. * p < 0.05 shows significant effects of H₂O₂ compared to untreated control values.