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. 2022 Dec 15;17(12):e0278848. doi: 10.1371/journal.pone.0278848

Fig 4. Skin melanocytes express TRPML3.

Fig 4

(A) Images showing sections of the back skin. τGFP expression is visible in hair follicles (HF). (B) Trpml3 levels in back skin correlate with initial synchrony of the hair follicle growth cycle. Follicle growth phases (anagen I, P9; anagen II, P27) show highest Trpml3 levels and the intervening follicle destruction phase (catagen/telogen, P20) shows the lowest Trpml3 level. Trpml3 levels detected and normalized similar to Fig 1 and displayed relative to normalized Trpml3 levels in P9 back skin. (C-E) In situ hybridization on sections of CD1 (white) back skin using 5’ (C,E) and 3’ (D) probes for showing the presence of Trpml3 in anagen I (P10; C, D) and anagen II (P27; E) hair follicles. (F, G) BrdU immunohistochemistry performed with in situ hybridization shows Trpml3 expressed in non dividing cells of anagen 1 (P6) hair follicles. (H-O) Immunohistochemistry on sections of P4-P7 (anagen I) Trpml3+/+ (H, L, N) and Trpml3-/- (I) skin using an antibody raised against the amino-terminus of TRPML3 (NT) labels tyrosinase positive melanocytes in Trpml3+/+ hair follicles (H, J, L, M) and glabrous skin (N, O) but not in Trpml3-/- follicles (I, K). H-K represent images from pigmented mice. Melanin was bleached to reveal immunoreactivity. L-O represent high magnification images from CD1 (white) mice of single melanocytes with dendritic processes indicated (arrowheads). Irregularly shaped objects on C-E and H-K indicate the dermal papilla. Scale bars indicate 10 μm in C-E and H-K; 50 μm in F, G; 2 μm in L-O.