Fig 8. TRPML3 distribution in salivary gland, adrenal gland, and pituitary gland.

(A) Overview image of the salivary gland with its lobules. Two submandibular lymph nodes (LN) are located at the sites. (B) Zoomed image of the upper lmph node (LN). (C) Zoomed image of the salivary gland tissue reveals the typical structure of secretory acini (arrowheads), consisting of several secretory cells. Some of these acini show τGFP expression. (D-F) Immunostainings show CgA expression in secretory cells. Some of the secretory cells building up one acinus reveal higher CgA expression than others. This higher CgA expression is colocalizing with τGFP (merged/yellow). (G) Immunostaining of the kidney with the adrenal gland (AG) sitting on top using CgA to label neuroendocrine cells. (H-K) Magnified images of the adrenal gland from (G). DAPI staining reveals the adrenal medulla (AM) and the adrenal cortex (AC). CgA antibody stains the catecholamine secreting chromaffin cells in the adrenal medulla. τGFP+ cells are nearly absent from the adrenal medulla, but seem to be more prominent in outer regions of the adrenal cortex. (L) Overview image of the pituitary gland. A, adenohypophyis. N, neurohypophysis. I, intermediate lobe. τGFP expression was mainly found throughout the adenohypophysis. (M-O) Zoomed image and immunostaining of the adenohypophysis using an antibody against CgA reveals several endocrine cells (CgA+ cells) overlapping with τGFP (arrowheads).