Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1991 Nov;50(11):787–791. doi: 10.1136/ard.50.11.787

Immunolocalisation of type VI collagen in the intervertebral disc.

S Roberts 1, S Ayad 1, P J Menage 1
PMCID: PMC1004559  PMID: 1772294

Abstract

Type VI collagen has been isolated from many connective tissues, including the intervertebral disc. Distribution of this collagen, however, varies considerably within different tissues. In adult mammalian nasal and articular cartilage it is localised preferentially in the matrix immediately surrounding the cell. Intervertebral discs from various species and of various ages were studied and a similar pericellular localisation was found. When antisera to type VI collagen were used staining was seen around the cells of all sections of intervertebral disc, being particularly prominent in the nucleus pulposus. Staining on or around the cells was also seen in the adjacent cartilaginous end plate and bone.

Full text

PDF
789

Images in this article

788Image
on p.788
789Image
on p.789
789Image
on p.789
789Image
on p.789
790Image
on p.790
790Image
on p.790

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abedin M. Z., Ayad S., Weiss J. B. Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens. Biosci Rep. 1982 Jul;2(7):493–502. doi: 10.1007/BF01115247. [DOI] [PubMed] [Google Scholar]
  2. Aplin J. D., Charlton A. K., Ayad S. An immunohistochemical study of human endometrial extracellular matrix during the menstrual cycle and first trimester of pregnancy. Cell Tissue Res. 1988 Jul;253(1):231–240. doi: 10.1007/BF00221758. [DOI] [PubMed] [Google Scholar]
  3. Aumailley M., Mann K., von der Mark H., Timpl R. Cell attachment properties of collagen type VI and Arg-Gly-Asp dependent binding to its alpha 2(VI) and alpha 3(VI) chains. Exp Cell Res. 1989 Apr;181(2):463–474. doi: 10.1016/0014-4827(89)90103-1. [DOI] [PubMed] [Google Scholar]
  4. Ayad S., Evans H., Weiss J. B., Holt L. Type VI collagen but not type V collagen is present in cartilage. Coll Relat Res. 1984 Mar;4(2):165–168. doi: 10.1016/s0174-173x(84)80023-0. [DOI] [PubMed] [Google Scholar]
  5. Ayad S., Marriott A., Morgan K., Grant M. E. Bovine cartilage types VI and IX collagens. Characterization of their forms in vivo. Biochem J. 1989 Sep 15;262(3):753–761. doi: 10.1042/bj2620753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Becker J., Schuppan D., Benzian H., Bals T., Hahn E. G., Cantaluppi C., Reichart P. Immunohistochemical distribution of collagens types IV, V, and VI and of pro-collagens types I and III in human alveolar bone and dentine. J Histochem Cytochem. 1986 Nov;34(11):1417–1429. doi: 10.1177/34.11.3772076. [DOI] [PubMed] [Google Scholar]
  7. Bruns R. R. Beaded filaments and long-spacing fibrils: relation to type VI collagen. J Ultrastruct Res. 1984 Nov;89(2):136–145. doi: 10.1016/s0022-5320(84)80010-6. [DOI] [PubMed] [Google Scholar]
  8. Buckwalter J. A., Maynard J. A., Cooper R. R. Banded structures in human nucleus pulposus. Clin Orthop Relat Res. 1979 Mar-Apr;(139):259–266. [PubMed] [Google Scholar]
  9. Chu M. L., Conway D., Pan T. C., Baldwin C., Mann K., Deutzmann R., Timpl R. Amino acid sequence of the triple-helical domain of human collagen type VI. J Biol Chem. 1988 Dec 15;263(35):18601–18606. [PubMed] [Google Scholar]
  10. Cornah M. S., Meachim G., Parry E. W. Banded structures in the matrix of human and rabbit nucleus pulposus. J Anat. 1970 Sep;107(Pt 2):351–362. [PMC free article] [PubMed] [Google Scholar]
  11. Engvall E., Hessle H., Klier G. Molecular assembly, secretion, and matrix deposition of type VI collagen. J Cell Biol. 1986 Mar;102(3):703–710. doi: 10.1083/jcb.102.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Eyre D. R., Wu J. J., Apone S. A growing family of collagens in articular cartilage: identification of 5 genetically distinct types. J Rheumatol. 1987 May;14(Spec No):25–27. [PubMed] [Google Scholar]
  13. Keene D. R., Engvall E., Glanville R. W. Ultrastructure of type VI collagen in human skin and cartilage suggests an anchoring function for this filamentous network. J Cell Biol. 1988 Nov;107(5):1995–2006. doi: 10.1083/jcb.107.5.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ludowieg J. J., Parker J. The mammalian intervertebral disk: fibrous structures of the whale nucleus pulposus. J Cell Sci. 1973 May;12(3):925–932. doi: 10.1242/jcs.12.3.925. [DOI] [PubMed] [Google Scholar]
  15. Meachim G. Meshwork patterns in the ground substance of articular cartilage and nucleus pulposus. J Anat. 1972 Feb;111(Pt 2):219–227. [PMC free article] [PubMed] [Google Scholar]
  16. Poole C. A., Ayad S., Schofield J. R. Chondrons from articular cartilage: I. Immunolocalization of type VI collagen in the pericellular capsule of isolated canine tibial chondrons. J Cell Sci. 1988 Aug;90(Pt 4):635–643. doi: 10.1242/jcs.90.4.635. [DOI] [PubMed] [Google Scholar]
  17. Wu J. J., Eyre D. R., Slayter H. S. Type VI collagen of the intervertebral disc. Biochemical and electron-microscopic characterization of the native protein. Biochem J. 1987 Dec 1;248(2):373–381. doi: 10.1042/bj2480373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. von der Mark H., Aumailley M., Wick G., Fleischmajer R., Timpl R. Immunochemistry, genuine size and tissue localization of collagen VI. Eur J Biochem. 1984 Aug 1;142(3):493–502. doi: 10.1111/j.1432-1033.1984.tb08313.x. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES