Table 4.
Effects of treatments on the relative mRNA abundance of genes involved in growth in proliferating and fused myoblast cells a.
| Proliferating cultures a | ||||||||||
| Treatment | ||||||||||
| Time (h) | Con | TBA | Met | Orn | Put | Spd | Spe | SEM | ||
| Pax7 | ||||||||||
| 0.5 | 0.91 | 1.02 | 1.64 | 0.57 | 0.58 | 1.02 | 1.17 | 0.66 | ||
| 12 | 0.97 | 0.83 | 1.09 | 0.98 | 0.56 | 1.68 * | 0.67 | 0.29 | ||
| 24 | 0.96 | 0.96 | 1.59 | 0.98 | 2.94 * | 0.71 | 1.27 | 0.46 | ||
| Spry1 | ||||||||||
| 0.5 | 1.00 | 1.22 | 0.78 | 1.00 | 1.05 | 1.74 | 1.26 | 0.83 | ||
| 12 | 0.98 | 1.74 | 1.01 | 1.34 | 1.81 * | 0.42 | 0.70 | 0.44 | ||
| 24 | 1.00 | 1.55 | 1.27 | 2.08 * | 1.00 | 0.71 | 0.63 | 0.69 | ||
| MapK | ||||||||||
| 0.5 | 1.00 | 0.84 | 0.20 * | 0.75 | 0.66 | 0.96 | 1.03 | 0.53 | ||
| 12 | 1.00 | 0.70 | 1.32 | 0.86 | 1.08 | 0.62 | 0.76 | 0.33 | ||
| 24 | 0.93 | 1.08 | 1.96 * | 0.53 | 2.09 * | 0.71 | 1.49 | 0.47 | ||
| Fused cultures b | ||||||||||
| Treatment | ||||||||||
| Time (h) | Con | TBA | E2 | TBA + E2 | Met | Orn | Put | Spd | Spe | SEM |
| mTOR | ||||||||||
| 1 | 1.00 | 0.84 | 0.71 † | 0.71 | 0.83 | 0.56 * | 0.78 | 0.83 | 0.69 † | 0.15 |
| 12 | 1.00 | 1.20 | 1.20 | 1.45 | 1.08 | 1.10 | 1.87 * | 1.97 * | 1.31 | 0.27 |
| 24 | 1.00 | 0.33 | 1.04 | 1.45 | 1.30 | 1.34 | 0.79 | 1.20 | 0.96 | 0.33 |
| eIF-2Bε | ||||||||||
| 1 | 1.00 | 0.97 | 0.86 | 1.02 | 0.90 | 0.90 | 0.91 | 0.95 | 0.91 | 0.18 |
| 12 | 1.00 | 0.98 | 1.04 | 1.21 | 1.07 | 1.13 | 1.44 * | 1.28 | 1.31 † | 0.18 |
| 24 | 1.00 | 1.29 | 0.99 | 1.14 | 1.35 | 1.01 | 1.29 | 0.97 | 0.92 | 0.26 |
a Proliferating cultures: Relative mRNA abundance of paired box transcription factor 7 (Pax7), Sprouty1 (Spry1), and mitogen-activated protein kinase (MapK) in proliferating C2C12 and Sol8 murine myoblast cells 0.5 h, 12 h, and 24 h after treatment with 1% fetal bovine serum (FBS, control, Con), 10 nM trenbolone acetate (TBA), 10 mM methionine (Met), 8 mM ornithine (Orn), 3 mM putrescine (Put), 1.5 mM spermidine (Spd), or 0.5 mM spermine (Spe). b Fused cultures: Relative mRNA abundance of mammalian target of rapamycin (mTOR) and eukaryotic translation initiation factor 2B subunit epsilon (eIF-2Bε) in fused C2C12 and Sol8 murine myoblast cells 1 h, 12 h, and 24 h after treatment with serum-free media (SFM, control, Con) as a control. The other treatments were similar to those described in the proliferation assay with the exception of the addition of 10 nM TBA + 10 nM E2 (TBA + E2). Values with a star (*) indicate a difference (p ≤ 0.05) whereas a cross (†) indicates a tendency (0.05 < p ≤ 0.10) for difference in relative mRNA abundance compared to control cultures within a time point.