Table 1.
List of strains used in the present work. Promoter sequences have been described previously [32]. ahpC* denotes a mutant ahpC enzyme [28,29].
| Strain | Genotype | Citation |
|---|---|---|
| Shuffle® T7 | F’ lac, pro, lacIq/Δ(ara-leu)7697 araD139 fhuA2 lacZ::T7 gene1 Δ(phoA)PvuII phoR ahpC* galE (or U) galK λatt::pNEB3-r1-cDsbC (SpecR, lacIq) ΔtrxB rpsL150(StrR) Δgor Δ(malF)3 | [25] |
| SBHS016 | A19 ΔtonA ΔtnaA ΔspeA ΔendA ΔLAM ΔsdaA ΔsdaB ΔgshA Δgor RF1(N296K/L297R/L298R) araB::T7 galK::Pc0-2XDsbC met+ | [6] |
| SBDG175 | SBHS016 ΔompT araB::T7 galK::Pc0-2XDsbC | this work |
| SBDG404 | SBSH016 araB::T7 galK::Pc0-2XDsbC tnaA::Pc0-ahpC* trxB::Pwt-gshA | this work |
| SBDG407 | SBDG403 ΔtrxA | this work |
| SBDG414 | SBHS016 lacZ::Pmtl-2xlDsbC | this work |
| SBDG417 | SBDG404 lacZ::Pmtl-2xlDsbC | this work |
| SBDG419 | SBDG407 lacZ::Pmtl-2xlDsbC | this work |