Table 2.
Comparison of product quality between ADCs of aCD74 and ADC-X produced in CFPS reactions with a HC/LC co-expression format or a HC expression with PFLC reagent format. Analytical SEC results report the relative amounts of High Molecular Weight (%HMW), Monomer, and Low Molecular Weight species (%LMW) by integration of the HPLC chromatogram at 280 nm. Conjugation efficiencies by MALDI-TOF MS are reported as Drug Antibody Ratio (DAR) and conjugation efficiency (% conj).
| aCD74 ADC | ADC-X | ||||
|---|---|---|---|---|---|
| Analytical SEC | % HMW | 1% | 1% | 1% | 2% |
| % Monomer | 95% | 98% | 96% | 95% | |
| % LMW | 4% | 1% | 3% | 3% | |
| MALDI-TOF MS |
DAR | 1.9 | 1.9 | 3.9 | 3.9 |
| % conj | 95% | 95% | 98% | 98% | |