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. 2023 Feb 28;12(3):601. doi: 10.3390/antiox12030601

Figure 2.

Figure 2

GmMPK6 increases salt tolerance. (A) The phenotype of NaCl with MPK inhibitor or NADPH-oxidase inhibitor-treated soybean seedling. Four-day-old seedlings were treated with 0.15 M NaCl and with or without 30 μM SB202190 or 50 μM DPI for 3 days. B & D solution-treated soybean seedlings were used as a control. Bar = 2 cm. (B) The chlorophyll contents of 0.15 M NaCl and inhibitors, respectively, co-treated soybean leaves. Ten-day-old soybean seedlings were treated with 0.15 M NaCl, 30 μM SB202190 + 0.15 M NaCl, and 50 μM DPI + 0.15 M NaCl for 5 days, respectively, and then chlorophyll contents were measured for leaves. B & D-solution-treated soybean seedlings were used as a control. (C) The effect of SB202190 on the expression of GmRD19A in NaCl-treated soybean seedlings. Seven-day-old seedlings were treated with 30 μM SB202190 for 1 h, followed by the treatment of 0.3 M NaCl for designated time points. For RT-qPCR, total RNA was isolated, and the amplification was carried out with gene-specific primers (Table S1). (D) Schematic diagram of GmMPK6-dependent ROS generation through the induction of GmRbohI1. Data are means ± SD of three repeats: **, p < 0.01.